Highly customizable low volume. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. The dual monochromator optics allow. 5 87. The system uses two scanning monochromators to determine the optimal excitation and emission settings. SpectraMax Spectrofluorometers. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. , April 14, 2022 – Molecular Devices, LLC. Positions H21-K22 contained plate blanks of PBS only. The SpectraMax® ABS Plus Microplate Reader can run . Tecan Infinite Microplate Reader. The powerful analysis tools in the software generate a standard curve and calculated data. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The guide also covers the software features, the specifications, and the accessories of the systems. Introduction. 58% for the detection module and 2. no. Add to Cart. In addition, the SpectraMax® iD5 can be expanded to include bottom. Offer Ends In: 18 Days 19: 38: 21. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 306 plate air cal fail,low light. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Please refer to the Obsolete Microplate Reader Models for more information. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. We would like to show you a description here but the site won’t allow us. The screw remains inside the instrument. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. 0 out of 5 $ 5,999. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. A new technical approach to platelet aggregation studies. 3) This warranty. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Figure 1. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 1. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. 315 can't find zero order. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. . microplate reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. A: Microplate Preparation. 0 out of 5 $ 9,995 $ 9,495. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. . The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Fully tested with validations, warranty. Table 1. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Also see:. 2 only: SpectraMax Mini Update. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Table 1. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Offer Ends In: 2 Days 10: 40: 19. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. 305 reference level saturation. Figure 3. Ease of use. In addition, the iD5 reader measures TRF and FP. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. . This unlocks the transport slide. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Table of Contents. For readers without a cuvette port, these options are not shown in the software. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. Total volume (except deep. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Temperature control. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The calculated LOQ values were 0. Additionally, user-installable options allow the SpectraMax i3 System to. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. Step 2: Launch SoftMax Pro Software. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Our absorbance plate readers feature our PathCheck Sensor technology and. An assay plate containing a 15-point standard curve was read on the SpectraMax. Microplate readers’ standard detection modes. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. . 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. SpectraMax® Microplate Readers and SoftMax® Pro Software. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. 58% for the detection module and 2. Table 2 summarizes the results for the uniformity test. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The calculated LOQ values were 0. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices SpectraMax M2E Microplate Reader. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. For readers without a cuvette port, these options are not shown in the software. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. These lab workhorses are used in drug discovery. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Endpoint, kinetic, spectrum. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. AlphaScreen. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. SpectraMax Microplate Readers. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. . Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. 7°C and the bottom to 36. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Devices’ StakMax® Microplate Stacker in a matter of minutes and . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Download PDF. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Assays vary depending on. Compact designs. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. With optimized reagents, option to operate the. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The advanced optical and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax ® M2 Multi-Mode Microplate Reader. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Figure 1. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 3) At the bottom of the strip, click on "Show. microplates or in a cuvette at a. . on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. 72 MB. . Three low volume 24-well bottom slides. Microplate Readers. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 313 reference gain check fail. Each sample has a discrete sample beam and reference beam so that each well is measured directly. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Wide range of applications. Thank you!. zip. More Product Information. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Contact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax M2 and M2e are versatile and reliable. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The 2-channel configuration is available with 0, 2, or 4 injectors. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Reader modes are customized to your specific applications with options to upgrade easily. 304 signal level saturation. 7°C (gradient heating design). Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. With optimized reagents, option to operate the. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. We would like to show you a description here but the site won’t allow us. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Step 2: Launch SoftMax Pro Software. limit of detection (LLD) for ATP was estimated for each microplate reader using the . 2. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 1. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Nucleic Acid and Protein Quantitation. Molecular Devices: Molecular Devices. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. SoftMax® Pro 7. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Next generation SpectraMax® absorbance microplate readers. Unparalleled performance on a personalized platform. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 100 readings per well gives optimal Z’ values for this assay. This pathlength correction is accomplished only with the use of. Use the provided 3. Learn more about its features, specifications, and applications in this user. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Bookings are free, but you will need to use iLab to book. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. flexibility of monochromator-based systems. The CellTiter-Glo assay from Promega uses luciferase. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 5 12. When the user applies a set temperature of 37°C, the top is heated to 37. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. We would like to show you a description here but the site won’t allow us. Products : General, Microplate Readers. 020 fmol 0. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. 2 only) SpectraMax M-series Microplate readers. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Published Dec 16, 2020 | Updated Dec 16, 2020. The plate reader can measure multiple readouts, including UV and visible absorbance. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Transcreener ® ADP. com. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. To resume collecting kinetic measurements within the same file, press the Append button. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. Download PDF. both cuvette-based and microplate reader applications on the same instrument. Access this by going to Help >. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 7°C (gradient heating design). Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Plate chamber design of the SpectraMax category 1 microplate readers. 100 fmol 1536-well (8 µL) 0. . The plate reader optics are tunable for. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. 314 low lamp level warning. limit of detection (LLD) for ATP was estimated for each microplate reader using the . The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Create custom fluorescence assays without having to purchase expensive filter sets. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. flexibility of monochromator-based systems. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 2 software: see SoftMax® Pro 7. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. With optimized reagents, option to operate the. Selection of Water Constant in PathCheck settings. The SpectraMax® ABS Plus Microplate Reader can run . Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Fill a clean plate with water. . When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. ATP standard curves read on SpectraMax microplate readers. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 40266 Email: drugdiscoverytech@lifetech. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 003 fmol 0. e. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Summary of Contents for Molecular Devices SpectraMax M2. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. Compact and tunable absorbance microplate readers DATA SHEET. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Step 1: Turn on your microplate reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. MPN:SpectraMax Plus. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. SpectraMax Microplate Readers. 5 12. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Align the two slots on the bottle holder with the two pegs on the rail. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments.